《植物生理学报》 2014, 50(6): 801-804

粗缩病对不同抗性玉米品种叶片生理特性的影响

穆西玉¹, 张海艳^1,2,*, 赵延明^1,2

¹青岛农业大学农学与植物保护学院/山东省旱作农业技术重点实验室, 山东青岛266109; ²山东省小麦玉米周年高产高效生产协同创新中心, 山东泰安271018

通信作者：张海艳；E-mail: hyzhang608@126.com；Tel: 0532-86080447

摘　要：

以6个对玉米粗缩病(MRDV)表现不同抗性的玉米品种为材料, 研究了粗缩病对玉米叶片叶绿素含量、超氧化物歧化酶(SOD)活性和可溶性蛋白含量的影响。结果表明, 感病后, 各品种叶片叶绿素含量、SOD活性和可溶性蛋白含量显著降低, 叶绿素含量和SOD活性下降幅度表现为感病品种>中抗品种>抗病品种, 可溶性蛋白含量下降幅度表现为抗病品种>中抗品种>感病品种(‘青农105’除外)。对病情指数与各生理指标变化幅度的相关分析发现, 叶绿素含量和SOD活性的下降幅度与病情指数均呈显著正相关, 除‘青农105’外的5个品种可溶性蛋白含量的下降幅度与病情指数呈极显著负相关。这说明, 品种对粗缩病的抗病性与感病后各生理指标的变化幅度有关; 品种抗性越强, 感病后叶绿素含量和SOD活性下降幅度越小, 可溶性蛋白含量下降幅度越大。

关键词：玉米; 粗缩病; 叶绿素; 超氧化物歧化酶; 可溶性蛋白

收稿：2014-01-13   修定：2014-04-29

资助：青岛市科技计划项目[11-2-4-5-(8)-jch]、国家自然科学基金项目(31101100)、作物生物学国家重点实验室开放课题项目(2013KF05)、山东省农业科技成果转化资金(2011)和山东省旱地作物水分高效利用科研创新团队。

Effects of Maize Rough Dwarf Virus (MRDV) on Physiological Characteristics in Leaves of Different Maize (Zea mays) Varieties

MU Xi-Yu¹, ZHANG Hai-Yan^1,2,*, ZHAO Yan-Ming^1,2

¹College of Agronomy and Plant Protection, Qingdao Agricultural University/Shandong Key Laboratory of Dry Farming Technique, Qingdao, Shandong 266109, China; ²Cooperative Innovation Center of Efficient Production with High Annual Yield of Wheat and Corn, Tai’an, Shandong 271018, China

Corresponding author: ZHANG Hai-Yan; E-mail: hyzhang608@126.com; Tel: 0532-86080447

Abstract:

Six maize (Zea mays) varieties with different resistance to maize rough dwarf virus (MRDV) were used to study the effects of MRDV on chlorophyll content, superoxide dismutase (SOD) activity and soluble protein content in leaf. The results showed that three physiological characteristics decreased significantly after the plants were infected by MRDV. The decline of chlorophyll contents and SOD activities appeared susceptible varieties>moderate resistant varieties>resistant varieties, and the decline of soluble protein contents appeared resistant varieties>moderate resistant varieties>susceptible varieties (except ‘QN105’). Correlation analysis indicated that the decline of chlorophyll contents and SOD activities were positively correlated with the disease index. The decline of soluble protein contents in five maize varieties except ‘QN105’ were negatively correlated with disease index. Therefore, the resistance to MRDV was related to the decline range of every physiological characteristic. The stronger resistance, the less decline of chlorophyll content and SOD activity, the greater decline of soluble protein content.

Key words: maize; MRDV; chlorophyll; SOD; soluble protein